FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11377143
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8000.020.1461661
ACACGTC11200.014.19429413
CGTCGAA759.873698E-414.0583831
ATCGGAA11950.013.5545662
TCGGAAG11800.013.345573
ACGTCTG12550.012.66791915
CGTCTGA12800.012.65473716
CGGAAGA12750.012.46882254
CACGTCT14050.011.63572114
AACGGGA21200.011.5312358
AAACGGG26900.011.2621987
CGTTGGA24950.010.9875141
ACGGGAT17250.010.7810359
GAAACGG27750.010.7550936
CGGGATT16700.010.68706310
GCACACG15150.010.59251411
GGAAACG27300.010.2730465
AACGGGT5350.010.0918968
CCACGAT3900.010.0417021
CCCGAAA4250.09.9235641
Produced by FastQC (version 0.11.5)