FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10707223
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC108970.10177242035586632TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19250.027.58190744
CTCGTAT19350.027.55563542
TCTCGTA19400.027.36915841
TCGTATG20400.026.24761243
ATCTCGT21400.024.91642640
CATCTCG22450.023.7515139
GATCGGA25150.021.340141
GTATGCC26050.020.64111745
ATCGGAA29100.018.8685822
ACACGTC29150.018.4457513
TCGGAAG30700.017.8119033
CGTCTGA30600.017.79225216
ACGTCTG31600.017.08681515
CACGTCT32850.016.7790614
TATGCCG5850.016.53695945
CGGAAGA33700.016.3589064
GCACACG34150.016.27216111
CACACGT36450.015.24538512
GATCATC38750.013.99377836
CGTTGGA28600.013.4830561
Produced by FastQC (version 0.11.5)