FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7391258
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22900.013.6952871
AAACGGG21700.012.7478377
ACGGGAT14050.012.6456719
AACGGGA18500.012.399948
CGGGATT13900.011.97305910
GAAACGG23100.011.7840286
ACCGCGC6600.011.58729231
CACCGCG6900.010.7575130
GGAAACG25150.010.6745775
CGGGATA3401.4093712E-79.922029510
CGCGCCT3206.554819E-79.84067833
CCGCGCC7250.09.61768732
ACGCCCG5101.10958354E-109.26181434
CCCCGAA3003.1190048E-59.0250961
GCCACCG17900.08.79560128
CCCGTAA3603.116822E-68.7743991
AACGGGT5151.3023964E-98.7340148
CCCGAAA3104.5855762E-58.7339631
CGTGCAA2606.8163994E-48.6779771
ACAGGCG28350.08.6470418
Produced by FastQC (version 0.11.5)