FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10945361
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG18500.011.59454422
GATCGGA3800.010.6628181
ACGGGAT15150.010.5935799
CGTGAGC20500.010.53654123
GAGCCAC51750.010.31874926
ACAGGCG27800.010.30528818
CGTTGGA23850.010.1933851
AACGGGA21400.010.1633228
CCCAAAG88700.09.9651421
CCCTAAA26600.09.7601451
AAACGGG25850.09.7484197
GAAACGG26500.09.679166
GCCACCG20350.09.6558928
ATCGGAA4900.09.4904062
CCCAAGT35250.09.3660261
CCCCGAT3552.5465852E-119.3000681
TACAGGC67400.09.27999517
CCCGAAT6150.09.2725741
CGGGATT14750.09.25381810
GGATTAC75600.09.20593213
Produced by FastQC (version 0.11.5)