FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7954059
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5350.023.12646142
TCTCGTA5550.021.88774541
GATCGGA5550.021.5261231
CGTATGC6000.020.62109244
TATCTCG5950.020.03822139
TCGTATG6750.018.3298643
ATCTCGT7200.016.55936440
ATCGGAA7600.015.7074662
ACACGTC8650.014.04358313
TCGGAAG8850.013.9979123
CGTTGGA25850.013.8649881
CGTCTGA9550.013.42677516
AACGGGA24150.013.32045658
ACGGGAT17450.012.7626269
AAACGGG30750.012.6561387
ACGTCTG10700.012.40419215
GAAACGG31850.012.2254926
CGGGAAT9600.012.18519110
CGGGATT16200.012.08104410
GGAAACG31600.011.4757925
Produced by FastQC (version 0.11.5)