FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6709201
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGG21100.013.7521357
GAAACGG21800.013.4142356
AACGGGA17750.013.3061938
CGTTGGA21700.012.9918291
ACGGGAT13800.012.7138889
CGGGATT12550.012.18633210
GGAAACG22850.012.0415025
AACGGGT4500.011.4968338
ACCGCGC5250.011.13862631
CACCGCG5850.010.38067230
CGGGTAT2201.3168513E-410.2231610
CGCGCCC3858.072675E-99.93129233
CCGCGCC6200.09.79466632
ACCACGC12550.09.67759831
CGGGAAT8200.09.59979710
GCCACCG12900.09.24074428
CCCCGTA2000.0069135249.0215261
AGGCGCG2050.0086553658.77761220
ATAGGCG3401.4970499E-58.60012218
CCCGGAT3451.7386328E-58.4985391
Produced by FastQC (version 0.11.5)