FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8434766
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA19600.012.2742221
ACGGGAT11450.011.6583539
AACGGGA16850.011.4826588
AAACGGG20400.011.2490017
GGAAACG20550.010.9479325
GAAACGG21250.010.8696226
GCGTGAG13050.010.803637522
ACAGGCG16600.010.57134918
CGTGAGC13200.010.56749323
CGGGATT11250.010.399075510
CAACGAC1602.7072467E-510.3371971
CCCAAAG69700.09.4271141
CCCAAGT26650.09.2528751
CCCAAAA33900.09.1368731
GAGCCAC37950.09.12983926
AGGCGTG16500.09.09010120
CCCTAAA20900.08.8488921
AACGGGT4105.456968E-128.7797078
CCGAGTA9050.08.6180182
CCTCGAT2803.1040872E-78.5919561
Produced by FastQC (version 0.11.5)