FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7527833
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23200.013.9980581
CGGGATT14250.013.57731710
AAACGGG24950.012.8942447
AACGGGA21300.012.56883058
ACGGGAT15850.012.4906149
GAAACGG26250.011.7455836
TCTCGTA3501.364242E-1011.57034843
GGAAACG26850.010.8960935
TATCTCG4202.7284841E-1110.71285741
CGAAACG1903.864934E-410.6600865
GATCGGA3205.5750206E-810.571451
AACGGGT5201.8189894E-129.9506648
CTCGTAT3806.949449E-89.47279944
CGGGAAT9650.09.09217410
GTTGGAA43700.08.9617232
TTTGCGG2350.0027115898.6159887
ATCGGAA4458.41701E-88.59832
GCGTGAG19450.08.32926722
CGTCTGA5453.748937E-98.25545216
CGTGAGC19350.08.02346523
Produced by FastQC (version 0.11.5)