FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7392798
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20900.013.9982751
ACGGGAT14450.012.7678489
AAACGGG23850.012.0754157
AACGGGA20350.011.7198478
GAAACGG24650.011.5921596
GGAAACG24650.011.41037755
CGTGAGC16100.011.04039623
CGGGATT13600.010.91884510
GCGTGAG16300.010.76689322
ACTGCGC6400.09.49175831
ACAGGCG23250.09.09611618
CCCCGAT2801.497994E-48.8412171
GCCACCG16600.08.80996128
CCCGGAT2851.8151465E-48.6861091
ACGCCCA6259.094947E-128.63972734
AGGCGTG22750.08.50493720
ACCACGC14550.08.50476931
CCCAAGT35700.08.4472251
CGGGAAT8350.08.35308510
GAGCCAC50100.08.30851326
Produced by FastQC (version 0.11.5)