FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5014754
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG2700.019.99953833
CGTACGT2750.019.6359134
CTCGTAT3200.016.17311544
ACCGTAC3350.016.11903232
CGTAATC3700.015.20280638
TCTCGTA3450.015.00100143
GTTCGAC900.003757341314.99935529
TCGTATG3700.014.59571345
GTACGTA3850.014.02565135
CCCCGGT1951.8252831E-713.85094451
GATCGGA4100.013.724261
ACGTAAT4250.013.23538537
AAACGGG15500.013.0640857
ATCTCGT4000.012.93862142
CCGCGCC4250.012.70558932
AACGGGA12900.012.55768
ACGGGAT10650.012.4642539
CGTATGC2351.3038698E-712.44776345
CGTTGGA15450.012.3829241
CGCCCGG6300.012.14257735
Produced by FastQC (version 0.11.5)