FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5219760
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA15150.013.3626248
AAACGGG17950.013.2832177
ACGGGAT11450.013.1622819
CGGGATT11000.012.47380610
GAAACGG19800.012.2711996
CGTTGGA18200.011.8896631
GGAAACG20950.011.1886425
CGCGCCC3754.5656634E-1010.79733
CGCCCGG7200.09.99779735
CGAGTCA1950.00573897559.22820528
ACCGCGC4702.3246685E-99.09321831
CGGGAAT6251.8189894E-128.99749910
CCGCGCC5007.4396667E-108.99749932
CCCGATG2300.00219208588.820321
GCGCCCG5701.09139364E-108.68229734
CGAAGAA4203.130408E-78.5869791
CCCGCAA2658.316501E-48.505971
CCCATAA16700.08.5034231
CACCGCG5058.434654E-98.46299630
GCCCGGC14100.08.29604436
Produced by FastQC (version 0.11.5)