FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12353564
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA37150.013.6960531
ACGGGAT24650.013.0517199
AACGGGA33500.012.558728
AAACGGG39750.012.16772657
CGGGATT23050.012.005568510
GAAACGG41550.011.6406066
GGAAACG42700.011.1687765
ACCGCGC9850.010.05266131
CGTATGC4501.0004442E-109.99978445
CACCGCG10950.09.45376530
CGCGCCC7950.09.34138933
CGGGATA5701.0913936E-119.07823810
CCGCGCC11050.08.9609732
CGCCCGG13000.08.65530735
CGGGAAT14200.08.55566910
ACTGCGC11200.08.439095531
ACAGGCG41100.08.15517418
GTTGGAA77100.07.9977172
CCCTAAA36750.07.8414871
GCGCCCA9900.07.72835834