FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7320175
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT12550.013.8018619
CGTTGGA19800.013.0900051
AACGGGA17450.012.633438
AAACGGG20200.012.4726087
CGGGATT12550.012.18865610
GAAACGG21400.011.8795456
GGAAACG22550.011.2922285
ACGGCTA3359.858923E-910.74399632
CGTATGC3857.221388E-1010.51725544
TCTCGTA3704.1927706E-910.3356541
CGGCTAC3704.1927706E-910.3356533
CCCGGAT2901.9344316E-610.103051
GCGTGAG19800.09.88427222
CTCGTAT4355.9517333E-99.30837442
CGTGAGC20500.09.21756223
TGCGCCC4752.8103386E-98.998095533
ACAGGCG26850.08.88080218
CGCGCCC4102.2353561E-78.7786333
AACGGGT4152.690158E-78.6728638
CGGGATA2952.6483633E-48.38805510
Produced by FastQC (version 0.11.5)