FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11029294
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGAG19100.012.09431122
CGTTGGA23750.012.0059471
CGTGAGC19150.011.90607423
CGGGATT13300.011.27769810
ACGGGAT15750.011.1423659
AACGGGA20450.011.0021598
AAACGGG26400.010.9656737
ACAGGCG25900.010.8875518
ATAGGCG4000.010.8745218
CCCAAAG93750.010.4051531
GAGCCAC53900.010.29676426
GAAACGG27050.09.8704436
CCCTAAT16800.09.8262971
AGGCGTG25750.09.72812220
TAGGCGT4350.09.65496419
CCCTAAA26900.09.5958321
CCCGAGT18300.09.5129091
GGAAACG27300.09.4505185
CCCAAGT35350.08.83039951
CAGGCGT26000.08.82673419
Produced by FastQC (version 0.11.5)