FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10352379
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC15150.033.11778644
CTCGTAT15550.032.4104242
TCTCGTA15950.031.59761641
TCGTATG16450.030.63720343
ATCTCGT17600.028.89101240
GATCGGA17150.028.3640291
ATCGGAA18700.026.112352
TCGGAAG18650.025.9410443
CGTCTGA19950.025.60046616
ACACGTC20550.024.63403713
AATCTCG21650.023.69430439
CGGAAGA21550.022.6589744
GTATGCC22350.022.54963945
ACGTCTG22650.022.54875615
GCACACG23350.021.87298611
CACGTCT24450.020.88872314
CACACGT25350.020.1472112
GTCACAC36450.013.88832629
AGAGCAC40900.013.0925058
AGCACAC43300.012.78251510
Produced by FastQC (version 0.11.5)