FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8954133
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3000.013.5271531
ATCGGAA3600.012.09412
CGTTGGA22000.012.0924551
ACGGGAT15700.011.5583419
GTCACCG3750.011.19789829
AACGGGA19600.011.1713728
CGGGATT14400.011.03959610
AAACGGG23800.010.9643297
TCACCGA4150.010.84139930
GAAACGG24250.010.6990236
GGAAACG23950.010.5907865
ACACGTC4150.010.4799613
TCGGAAG4050.010.3767113
CGTCTGA3950.09.87156616
CCCAAGT30800.09.0278621
ACGTCTG4900.08.87588515
CGGGATA3705.875336E-108.51191610
GCGTGAG16950.08.49398122
CCCTAAT13100.08.4903251
CCCAAAG76250.08.476031
Produced by FastQC (version 0.11.5)