FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7447940
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22650.012.9415021
ACGCCCG3950.012.53387834
ACGGGAT14150.011.9232759
AAACGGG22700.011.8917597
CGGGATT14300.011.48358810
AACGGGA18900.011.3071368
GAAACGG23550.011.1759836
ACCGCGC5150.010.92310231
GGAAACG25200.010.8013615
CGCCCGG7600.010.65965135
CGGCTAA7350.010.40910939
CACCGCG5600.010.04582530
GCCACCG15800.09.68468428
CGCGCCC4304.8457878E-99.42028233
ATCGCGC2658.262876E-59.3376497
CGTGAGC19350.09.30054923
CCGCGCC5851.8189894E-129.23241332
AAGCGGC5403.6379788E-119.1650983
GATCGCG2751.2373117E-48.9980996
GCGTGAG19550.08.86031722
Produced by FastQC (version 0.11.5)