FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5721052
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGT3500.012.8483468
CGTTGGA15050.011.8702941
GCGTGAG14800.011.69940122
ACAGGCG20400.010.80142918
AGGCGTG18450.010.72436520
CGATAAA2554.411824E-610.6417241
GAAACGG16300.010.4863686
CGTGAGC16400.010.42087823
AAACGGG15650.010.3446267
CAGGCGT19000.010.17724319
CGGGATT9000.09.99315710
GGAAACG16300.09.9814865
ACGGGAT9700.09.96740159
AACGGGA12250.09.911588
ACCGCGC4501.0659278E-99.49383231
GCCACCG12900.09.41331228
ACGCCCG2902.1764577E-59.304625534
GGCGTGA19100.09.29991621
CCCAAAG61650.09.2435411
CGGCTAA5607.2759576E-129.23555639
Produced by FastQC (version 0.11.5)