FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7177231
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23450.013.4676191
CCCCGAT1852.1880212E-512.1936551
AAACGGG23250.012.1915677
ACGGGAT14400.011.8737329
AACGGGA19250.011.4527648
GAAACGG25400.011.1594476
GGAAACG27000.010.7481035
CGGGATT13400.010.74564510
ACGGGTT5650.09.9546899
AACGGGT6450.09.7659228
CCCGTAA2352.4844692E-49.599261
ACGCCCG3003.2240496E-58.99972834
CCCGAAT8600.08.9183821
GATTTCG10000.08.7734525
CGCCCGG6603.6379788E-128.52187535
CGGGAAT8200.08.2312310
GTTGGAA42000.08.1965252
CCACGAA4701.95545E-78.1593711
ACCACGC14350.08.15329131
CACCACG16850.08.01173930
Produced by FastQC (version 0.11.5)