FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5283156
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16400.013.7612971
AAACGGG17400.012.7974467
AACGGGT3405.456968E-1212.5683768
ACGGGAT9950.012.2053579
GAAACGG17900.012.1886636
AACGGGA14500.012.0984878
GGAAACG18150.011.4373055
CGGGATT10550.010.87170110
ACGGGTT3451.5646947E-810.4299139
CGCGCCC3351.1353404E-710.06992533
CGTAGAA2251.595786E-410.03045751
GATTTCG6750.010.0283615
ACCGCGC4304.656613E-109.93731531
ACAGGCG19000.09.82436118
CGCCCGG5800.09.69374935
CCCGAAT5607.2759576E-129.2692171
CGTAAGC1950.00574255279.227509523
CGGCTAA6700.09.06404839
ACGCCCG3258.481033E-68.995834
CCGAGTA8900.08.8755172
Produced by FastQC (version 0.11.5)