FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7104968
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT13000.014.1840039
AACGGGA18600.014.1449438
AAACGGG23300.013.4147037
CGTTGGA23950.013.3003721
GAAACGG23800.013.1328816
CGGGATT12100.013.00900310
CGCCCGG6850.012.14719835
GGAAACG25650.011.8885565
CGTAAGA2707.488434E-710.8775181
CGTGAGC18900.010.82798423
GCGTGAG18600.010.51877422
CGGGAAT9150.010.07613810
GCCCGGC15000.010.04496836
ACGCCCG4152.6611815E-99.753942534
GATTTCG10200.09.7439545
CGCGCCC3858.553616E-89.34590133
CCTTACG1950.0055376919.2684171
ACCGCGC5352.910383E-119.24757331
AGGCGTA1950.0057502799.22608120
ACAGGCG27300.09.22517218
Produced by FastQC (version 0.11.5)