FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7212601
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5950.024.19173642
CGTATGC6350.024.08458944
TCTCGTA6100.023.96605941
TCGTATG7300.020.02601443
GATCGGA7600.019.3000051
ATCGGAA8500.017.7874432
ATCTCGT8500.017.19893340
TCGGAAG9050.016.7064383
ACACGTC9100.016.55878313
CGTCTGA9150.015.97681816
AATCTCG9550.015.77896439
CGGAAGA10200.014.6016334
GTATGCC11200.013.88565745
ACGTCTG10600.013.79121415
GCACACG11200.013.65481811
CACGTCT11600.012.9900814
CGTGAGC14800.011.54930723
GCGTGAG14750.011.13109522
CACACGT14200.010.92837912
CGTTGGA15300.010.7668611
Produced by FastQC (version 0.11.5)