FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7401430
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4900.020.19540242
CGTATGC5300.018.24687444
TCTCGTA5300.018.24687441
TCGTATG6050.016.72834843
GATCGGA6250.014.8065731
ATCTCGT6500.014.18697740
TCACGAT6400.014.05911635
ACACGTC7450.012.67911413
CGTCTGA7500.012.29471516
CGTTGGA22250.012.173121
TCGGAAG7650.012.0907253
ATCGGAA8050.012.0504282
CGGGATT14150.011.60278610
AAACGGG25200.011.6021567
GAAACGG25150.011.5382916
ACGGGAT15600.011.53350459
AACGGGA20750.011.0554898
ACGTCTG8800.010.98959415
GGAAACG25650.010.7300655
ACGATCT9350.010.58425737
Produced by FastQC (version 0.11.5)