FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9019860
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2700.016.1574921
ATCGGAA3100.014.5156272
ACACGTC2950.013.72689313
GTCACGC3600.012.49832129
TCGGAAG4300.011.5120593
CGTTGGA21150.011.3801861
CGTCTGA3650.011.09433916
GCGTGAG14800.010.64040422
ACAGGCG21600.010.4152118
AACGGGA18950.010.3689198
ACGGGAT13650.010.3291429
AAACGGG23950.09.8325377
AGTCACG4900.09.79460228
CGTGAGC15950.09.68516523
CGGGATT13450.09.47838610
GAAACGG25250.09.3866466
CCCAAAG71350.09.3400561
GGAAACG24700.09.2311155
CGGAAGA4900.09.1840184
CCCAAGT29750.09.1523221
Produced by FastQC (version 0.11.5)