FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4497746
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4950.028.17037842
TCTCGTA5050.027.61255341
ATCTCGT5450.025.58622740
CGTATGC5450.025.17326544
GATCGGA5650.024.3694081
ATCGGAA6000.023.6894112
TCGTATG6000.022.86571343
TCGGAAG6450.021.6856633
CGTCTGA6650.021.30713816
AATCTCG6600.020.78724539
ACACGTC6900.020.53514113
GTTACGC550.003947310620.4464720
CTCGCGT707.0069166E-419.2778857
ACGTCTG7900.018.2204515
GTATGCC7900.017.3967345
CGGAAGA8400.017.1886394
CACGTCT8150.017.10961714
GCACACG9050.015.90514311
CACACGT9700.014.839334512
CGATAGA1108.390788E-414.3637871
Produced by FastQC (version 0.11.5)