FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6108149
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18950.013.5564321
ACGGGAT10950.012.1222479
AAACGGG18600.011.97409257
AACGGGA15800.011.9603138
CGGGATT11550.011.68740410
GGAAACG20200.011.0288995
GAAACGG20000.011.0264016
CGCCAAT1900.0045650059.4882511
ACCGCGC5505.456968E-129.40797431
ACGCCCG3155.6802273E-69.28476534
ACCACGC11700.09.22967431
CACCGCG5901.8189894E-129.15250630
CGGGAAT6900.09.12972510
GCGTGAG14650.09.06065622
CGTGAGC14850.08.78712423
CGAGGAT3155.6126435E-58.5846071
GAGCCAC47100.08.45579526
GCCACCG14150.08.26899128
AACGGGT4406.5439235E-78.1806478
CACGCCC9100.08.15850633
Produced by FastQC (version 0.11.5)