FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7243449
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA5200.025.50881441
CTCGTAT5750.022.67580642
CGTATGC5900.022.48032844
ACGATCG500.002277488622.48001712
GATCGGA6150.022.1104451
TCGTATG6100.021.37473943
ATCGGAA7400.018.6737772
ATCTCGT7250.018.60607740
ACACGTC7700.017.51689513
TCGGAAG8200.017.4044673
CGTCTGA8250.016.89407316
CGGAAGA9600.014.6294274
ACGTCTG10100.014.24476215
AATCTCG10100.013.578444539
CGTTGGA20000.012.9180291
ACGGGAT13400.012.9176229
CACGTCT11450.012.368916514
AACGGGA18550.012.2397948
GCACACG11400.012.22597311
AAACGGG22750.012.154037
Produced by FastQC (version 0.11.5)