FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8023591
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC4250.014.82259233
ACCGCGC5600.013.2580931
AACGGGA17850.012.9829388
CACCGCG6100.012.909018530
ACGGGAT13650.012.856819
AAACGGG23000.012.8145677
CCGCGCC6350.012.75509732
CGTTGGA22250.012.5644481
GAAACGG24450.011.8706366
CGGGATT14150.011.60747310
GGAAACG26200.011.1635555
CGTGAGC18450.010.36519823
GCGTGAG18100.010.31702722
AACGGGT5900.010.2964078
GCCACCG17850.010.08339528
CGTTGAA3704.458161E-89.7492241
CCCCGAT2658.0657854E-59.3583351
ACAGGCG26050.09.32797218
CCACCGC12900.09.24360229
ACTGCGC7300.08.9377931
Produced by FastQC (version 0.11.5)