FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4423538
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGC53840.1217125296538653TruSeq Adapter, Index 18 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8150.031.74677745
CGTCCGC8100.030.55427432
TCTCGTA8250.030.27145842
GTCCGCA8250.029.99874133
ACGTCCG8300.029.8176931
CTCGTAT8600.029.03915243
TCCGCAC8550.028.94517334
TCGTATG9150.027.78540444
CGCACAT8850.027.45616536
ATCTCGT8900.027.30222541
CCGCACA8950.026.897435
CATCTCG9450.025.95129240
CACGTCC9950.025.55140330
TCACGTC10050.025.52102929
GTCACGT10700.023.76041628
AGTCACG10150.023.05295827
ATCGGAA11950.022.088041
TCGGAAG12650.020.9898722
ACACGTC12500.020.69796412
CGTCTGA12550.020.61503415
Produced by FastQC (version 0.11.5)