FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9957597
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC138400.13898935656865807TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19000.034.33788342
CGTATGC19600.033.40150544
TCTCGTA20050.032.76405341
TCGTATG20950.031.67868243
ATCTCGT22450.029.46185540
GATCGGA24250.027.4871791
GTATGCC25950.025.59596645
ATCGGAA26700.025.1336292
AATCTCG27300.024.47501239
TCGGAAG27700.023.9804613
ACACGTC29300.023.26540613
CGTCTGA29350.022.84216516
ACGTCTG31500.021.4976815
CGGAAGA31450.021.4049114
CACGTCT32000.021.1617814
GCACACG34450.019.8527211
CACACGT35450.019.29269812
GTAATCT39450.017.1651937
CCTTGTA42350.016.04313333
TATGCCG4150.015.73391545
Produced by FastQC (version 0.11.5)