FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6821603
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA18400.013.0835938
AAACGGG22700.012.88501557
GAAACGG24500.012.3994386
CGTTGGA19450.011.8063461
ACGGGAT13000.011.5955829
CGGGATT13200.011.41997510
GGAAACG25550.011.2744345
CCCGAAC2004.9549773E-511.256541
AACGGGT4501.0004442E-109.9995078
ACGGGAA8600.09.6797559
CGGGAAT8850.09.66061110
CCCGTGA4253.936293E-99.5349521
ACGGGTT4253.97813E-99.5289429
CCCGAAA4355.869879E-99.3157571
CCCGGTT3451.778645E-58.4831911
ACCGCGC5555.2514224E-98.10776731
GCTAGAC14450.07.940842629
CGTGGGA8201.8189894E-127.68739371
CGTATTC2700.0094137587.50210433
CGCGCCC5201.0212734E-67.355298533
Produced by FastQC (version 0.11.5)