FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12110537
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6500.014.53778245
CGTTTCG5900.013.72805732
CGTTGGA34200.012.3148681
CTCGTAT6600.012.27215343
AACGGGA30350.011.9354888
AAACGGG36750.011.7547467
ACGGGAT22350.011.6775959
GAAACGG38700.011.3365856
TCTCGTA7250.011.17189142
GATCGGA5950.010.9772951
GGAAACG39900.010.8264715
CGGGATT21300.010.66877910
CGCCCGG12800.010.54629435
TTCGGAA7350.010.4075935
GTTTCGG8250.010.36306633
ACGCCCG7000.010.28514934
TCGGAAT7850.010.03129536
CCTCGAT3653.6696292E-89.8728251
CGGGAAT13200.09.71569410
TCGGAAG9300.09.4448692
Produced by FastQC (version 0.11.5)