FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4541431
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA12950.013.3821671
ACGGGAT8750.012.8566789
CGGGATT8350.012.66421210
AAACGGG14350.012.07313257
AACGGGA12500.012.0595648
GAAACGG15700.011.7519436
CGCCCGG5650.011.548635535
GGAAACG15450.011.5051985
ACGCCCG3052.8472641E-811.06553834
ATCGCGC1800.00295653210.00007937
TGCGCCC3401.399676E-79.92643933
TCGCGCC2057.793515E-49.87812638
GCCCGGC10050.09.85082436
GCGTGAG11650.09.84942922
CCCGTGC2302.0476135E-49.7853781
ACTGCGC4001.499393E-89.56215431
CGTGAGC11600.09.31000823
CGTCAGT2000.00703333049.0025481
ACCACGC9050.08.94995231
CCACGAT3104.7208938E-58.7121441
Produced by FastQC (version 0.11.5)