FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7682516
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22100.012.3559531
AAACGGG25100.011.0249937
GAAACGG25600.010.8981846
AACGGGA19900.010.8533828
CGGGATT12550.010.57682310
GGAAACG26350.010.3318285
ACGGGAT14350.010.1908039
ACGGGTT5151.8189894E-1210.0477219
ACGCCCG4355.711627E-109.82678234
AACGGGT6350.09.5661468
CGGGAAT9450.08.57072710
CGATTTG3806.002497E-57.6971894
CGAGAAT4405.4804186E-67.69346051
CGGGTTT6451.3551471E-97.673790510
ACGGGAA9500.07.57832679
CCACGAA4507.533359E-67.5224951
CGCCCGG8105.456968E-127.499386335
CGGCTAA9150.07.37644539
CCACCGT9850.07.309046329
ACCGTGC8653.6379788E-127.282640531
Produced by FastQC (version 0.11.5)