FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7854737
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC127310.16208053815169113TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA18150.035.19996341
ACGGCTA18300.035.03436732
CTCGTAT18300.034.7885142
CGTATGC18500.034.41526444
CGGCTAC18800.033.9829433
GATCGGA19100.033.855531
TCGTATG19300.033.22080643
ATCTCGT19700.032.31602540
CATCTCG20150.031.48268739
GTCACGG20300.031.47188829
TCACGGC21400.029.85417630
ATCGGAA22100.029.6671812
ACACGTC22300.029.15313113
AGTCACG21800.029.1000128
CGTCTGA22600.028.66660316
TCGGAAG23000.028.6037063
CACGGCT22800.028.02102331
ACGTCTG23350.027.74583415
CGGAAGA23950.027.2797494
GTATGCC24000.027.11417845
Produced by FastQC (version 0.11.5)