FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6832495
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20600.013.8269141
AAACGGG23000.012.8072237
AACGGGA19000.012.54443558
GGAAACG24300.012.1772285
GAAACGG23650.012.1717776
ACGGGAT13600.012.069329
CGGGATT13100.011.32847610
CGGGAAT9400.010.04662810
CCCGAAA2301.9589216E-49.8286551
ACCGCGC5151.2732926E-119.60572631
GATTTCG9850.08.9435875
CCGCGCC5852.0008883E-118.84070132
CACCGCG5607.6397555E-118.833837530
CCTCGAA3603.0371884E-68.7911861
CCCCGGT2606.687779E-48.694581
GCGTGAG17400.08.52990922
CTCGTAT2658.511112E-48.48626442
CGTGAGC18150.08.42523523
AACGGGT5101.0128133E-88.376888
GCCACCG15550.08.24322928
Produced by FastQC (version 0.11.5)