FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12925232
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA39850.014.5902321
CGTACGT3350.014.10367733
AACGGGA35600.013.2098498
ACGGGAT26000.013.1544239
CCGTACG3650.012.9443232
AAACGGG43200.012.7078587
CGGGATT24150.012.4850210
CGTCCGA1454.8560117E-412.4133296
GAAACGG45050.012.0361776
GGAAACG46550.011.8421675
ACCGCGC9450.09.76123731
CGTATGC5800.09.69731345
CCCCGAA6000.09.389871
GTACGTA5302.3646862E-119.33909334
CGGGAAT16100.08.80473310
GCCACCG28000.08.6780828
CTCGTAT5251.8590072E-98.5707143
CACCGCG11300.08.56169330
GCGTGAG31250.08.42319622
CGGGATA5453.7362042E-98.25724310
Produced by FastQC (version 0.11.5)