FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12351005
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT16050.031.53229942
CGCCAAT16050.031.252933
TCTCGTA16300.030.91118441
CGTATGC16900.030.0794544
TATCTCG16850.029.90233439
ACGCCAA17150.029.37902332
TCGTATG17300.029.12393843
GATCGGA18450.027.277261
GTCACGC18550.027.16151829
ATCTCGT19600.025.93627440
ATCGGAA20250.024.8214632
ACACGTC20600.024.56794213
TCGGAAG21100.024.3545723
AGTCACG20700.024.12404628
CGTCTGA22150.022.84892316
ACGTCTG24050.021.41740415
CGGAAGA24700.020.5312544
CACGTCT26550.019.57045614
GTATGCC26600.019.39367345
GCACACG27650.018.54783211
Produced by FastQC (version 0.11.5)