FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9374796
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1191521.2709823232420205TruSeq Adapter, Index 6 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG351970.37544283630278463TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA130000.043.216551
TATGCCG53200.042.1818845
CGTCACG4700.033.9837427
CGCCAAT173350.031.89787733
ACGCCAA175900.031.48661432
TCTCGTA179500.030.84275841
AGTCACG180100.030.83977128
TATCTCG179500.030.83006139
ATCGGAA182500.030.7870332
CTCGTAT180100.030.72751842
TCGGAAG183000.030.7140773
GTCACGC180500.030.68418729
CGTATGC180800.030.6085544
TCGTATG181500.030.54008143
ACACGTC184400.030.53540413
ATCTCGT181450.030.52369940
CGGAAGA186200.030.3915824
CGTCTGA185950.030.20828816
ACGTCTG186300.030.15153715
CACGTCT187750.029.91867414
Produced by FastQC (version 0.11.5)