FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4318865
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA15600.016.0568241
CGAAACG1156.815777E-515.6450810
CGGGATT9050.014.66185910
ACGGGAT9000.013.9936549
AACGGGA13050.013.787018
AAACGGG15900.013.2966267
GAAACGG16850.012.94746
GGAAACG17550.012.2131325
ACGCCCG2351.7681468E-611.48428634
CGGGAAT5950.011.33939510
CACGCCC6650.010.14589233
CACCGCG3401.4083525E-79.922230
GCGGGAT1850.00372311759.7253199
GCTAGAC9950.09.72112429
GCGTGAG9750.09.68791422
GTCGGGA2352.5595172E-49.5706827
GCCACCG8250.09.54300628
CCCGTAA2150.00117354649.4463791
GTTGGAA30750.09.393582
ACCACGC7100.09.18618931
Produced by FastQC (version 0.11.5)