FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7275014
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8200.023.7288361
ACACGTC8950.021.25448213
CGTCTGA9200.020.67734116
ATCGGAA9650.020.1787782
TCGGAAG9800.019.5635553
ACGTCTG10050.019.07755515
CACGTCT10250.018.55917514
CGGAAGA10350.018.3792294
GCACACG10850.017.94640711
CACACGT11650.016.84260412
GTCACTA14100.014.03327729
TCACTAG14900.013.28008730
CGTTGGA16350.012.18631651
AGCACAC18150.012.04869810
ACGGGAT10100.012.0123399
CCCTAAA17650.011.9060941
AACGGGA14150.011.6439448
AAACGGG18250.011.4081667
GAGCACA18000.011.4001879
AGAGCAC17200.011.3208448
Produced by FastQC (version 0.11.5)