FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7001759
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGT4300.014.1260218
CGTTGGA19850.012.9556251
AAACGGG19500.011.42130857
GGCGCGC1400.00525948411.25034621
ACGGGAT12200.010.696219
GAAACGG21300.010.6671336
ACGGGTT4051.546141E-1010.5550519
CGGGATT11350.010.50663110
GGAAACG21150.010.4236955
CGTATGC3104.2306056E-710.1603744
CCCCGAT2302.0006283E-49.808131
CCGCGCC5301.8189894E-129.76396432
GATCGGA2555.2594627E-59.7312041
CACCGCG5550.09.72947330
AACGGGA16700.09.6993048
GATTTCG9350.09.6239725
ACCGCGC5151.2732926E-119.61146631
ACCACGC15150.09.50476731
GCGTGAG17500.09.25742822
TCTCGTA3206.9345497E-69.13999341
Produced by FastQC (version 0.11.5)