FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7251647
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT11100.012.97009210
CGCGCCC3202.910383E-1112.65483633
ACCGCGC4650.012.58114831
ACGGGAT12700.012.2216959
CGTTGGA20200.012.0836881
CACCGCG5150.011.79658830
AACGGGA17050.011.6103258
AAACGGG20950.011.1672027
CCGCGCC5050.011.13905932
GGAAACG22750.010.8816585
GAAACGG22150.010.5667256
CCCGAAT6800.010.3033841
CGCCCGG6150.010.2417735
ACGCCCA6400.010.19417334
GCCACCG14750.09.45800628
ACTGCGC6250.09.36037431
CACGCCC9700.09.27731433
CGTGAGC17050.09.1035523
ACAGGCG23350.09.05667918
CGCGCCT2250.00182699358.99899533
Produced by FastQC (version 0.11.5)