FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6055313
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA5650.025.07819441
CTCGTAT6000.024.73983642
CGTATGC5850.024.60527444
TATCTCG6350.023.0229939
TCGTATG6850.020.68493343
ATCTCGT7200.019.99195140
ATCGGAA7750.019.2133922
GATCGGA7650.018.586261
ACACGTC8700.016.28641313
TCGGAAG9300.015.5249453
CGTCTGA9600.014.52516416
GTATGCC10800.013.76822945
CGGAAGA11150.013.3537154
CGTTGGA20000.013.3157281
CACGTCT10950.012.93978214
AAACGGG20450.012.86768157
ACGTCTG10850.012.85175715
AACGGGA16700.012.5247958
GAAACGG21400.012.5067516
GGAAACG21650.012.0873065
Produced by FastQC (version 0.11.5)