FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4271597
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT8700.014.7367219
AACGGGA12300.014.629528
CGTTGGA12800.014.6247791
CGGGATT8350.014.00656310
AAACGGG14300.013.2126057
GAAACGG14750.012.6576086
GGAAACG15250.011.5349425
CGGGAAT5200.010.38060510
ACGGGAA5557.2759576E-129.3213779
CACCGCG3704.8043694E-79.11831230
CCCCGAA2050.0084637228.8015131
GTTGGAA24600.08.8015132
TCTCGTA2050.0086576298.77719941
AGCGGCT4651.7786078E-88.7311494
GATTTCG5201.4824764E-98.6739585
ACCGCGC3704.704447E-68.51042531
CCCAAAG48100.08.4401211
CCCTATA5352.5247573E-98.4313551
GCGTGAG10950.08.42209322
CGCCCGG5301.9987056E-88.06292235
Produced by FastQC (version 0.11.5)