FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6574291
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT12100.016.726499
CGTTGGA20500.014.654651
AACGGGA17200.014.6433558
CGGGATT12100.014.49629210
CTCGTAT3200.014.056396542
AAACGGG20350.013.923787
GAAACGG21600.012.9106676
GGAAACG21950.012.2398045
ACCGCAC5150.012.22686331
CCGCACC5450.011.14118832
GCGTGAG16400.011.10696722
TATCTCG4152.1827873E-1110.83866739
CGTGAGC16400.010.83272123
GCCACCG14600.010.62813828
CAGGCGT19200.010.54133419
ACAGGCG22450.010.31736118
CGGGATA3104.2566353E-710.15579810
TCTCGTA4001.4060788E-910.12060541
ACGCCCG3401.4108628E-79.92125634
CGGGAAT6650.09.80672710
Produced by FastQC (version 0.11.5)