FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7512997
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22350.015.1468721
ACGGGAT14900.013.891079
AAACGGG23650.013.7937567
AACGGGA19700.013.5898578
GAAACGG25700.012.8685596
CGGGATT14000.012.69501510
CACCGCG6250.012.23863730
GGAAACG26550.012.1176185
CCGCGCC6000.011.62370632
CGCGCCC4700.011.48808233
ACCGCGC5850.011.15260431
AACGGGT4803.274181E-119.8426548
CCCGATT3053.6198817E-69.6195051
CCCGTAA3401.4309735E-69.2930511
TCTCGTA3155.6810477E-69.28474241
CGGGAAT9250.09.24221410
CCCGAAT7700.09.0861561
CCCCGAA3309.84189E-68.8907551
CTCGTAT3053.9193386E-58.85153242
CGGGATA2607.015718E-48.65288310
Produced by FastQC (version 0.11.5)