FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5679915
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18000.015.5404811
AACGGGA16100.014.3896938
AAACGGG18650.013.9900527
ACGGGAT11800.013.7243169
CGGGATT10450.013.343616510
GAAACGG20450.012.8694366
ACGGGAA6850.012.14930259
CCCGAAA2054.696456E-612.1047021
GGAAACG21400.011.9108185
ACCGTGC4750.011.83728731
CGGGAAT7350.011.0157310
CGGCTAC2302.0620973E-49.77862832
ACGCCCG3258.1105463E-79.68836434
GATTTCG7350.09.513745
AACGGGT3858.528332E-89.3475868
GTTGGAA32550.09.2868622
CCCGTAA2200.00145224429.2285841
TATGCCG2000.00689531869.0243813
CCGTGCC6104.9112714E-118.48015532
ACCACGC10350.08.47481231
Produced by FastQC (version 0.11.5)