FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7516797
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC4100.015.35973233
ACCGCGC5800.014.34849431
CCGCGCC6050.013.754848532
CACCGCG6400.012.65188130
CGTTGGA23700.012.0006861
AAACGGG23750.011.4585917
CGCCCGG6900.011.40842135
AACGGGA21300.011.4039078
CGGGATT14150.011.28525510
ACGCCCG3052.8652721E-811.06109134
ACGGGAT14800.010.9415869
ACAGGCG24800.010.610620518
GAAACGG26200.010.3875666
GCGTGAG18850.010.38040922
GGAAACG26150.010.3518525
GCCACCG16100.010.33804528
CGTGAGC19450.010.29145923
GAGCCAC54700.09.66302526
CAGGCGT22850.09.44912519
GCCCGGC16850.09.34339536
Produced by FastQC (version 0.11.5)