FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335589
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA800.001716280916.86869615
CTCGTAT3850.016.35868644
TATCTCG3850.015.77419841
TCTCGTA4300.014.12340943
CGTTGGA17350.013.912071
ATCTCGT4550.013.34697642
TCGTATG4300.013.09863145
AAACGGG20150.012.3897157
AACGGGA16100.012.2933488
GGAAACG19350.012.2379675
GAAACGG19850.012.2371446
ACGGGAT11300.011.9422519
ACGGGTT4150.011.9230679
ACTGCGC5300.011.88291531
ACCGCGC3601.8189894E-1111.87112631
AACGGGT4850.011.1296858
CGCCCGG5550.010.537683535
CGGGATT10200.010.3636110
CGCGCCC3053.395453E-710.32441833
GATCGGA5900.010.3233161
Produced by FastQC (version 0.11.5)